Learning objectives: 1) Recognize the importance of education and training in suicide prevention for nursing assistant students 2) Assess the impact of flexible online training modules on nursing assistant students' knowledge of suicide and their willingness to intervene against suicidal behavior 3) Utilize the identification of key predictors of nursing assistant students' intention to intervene against suicidal behavior to inform future teaching strategies aimed at nursing assistant students Background: Nursing assistants frequently encounter individuals at risk of suicidal behavior in various healthcare settings. Despite this, they often lack systematic training in responding to suicidal behavior or implementing suicide prevention methods. This study aims to assess the impact of online, flexible suicide prevention training materials on nursing assistant students' knowledge of, and willingness to intervene in suicidal behavior. Materials: The training materials comprise four online modules featuring PowerPoint presentations, recommended literature, case studies, and simulation training. These modules cover risk factors, clinical tools for assessing suicidal risk, and treatment options. Additionally, an introductory module prepares teachers to deliver the materials and prompts self-reflection on attitudes toward suicide. Teachers have the flexibility to select relevant modules for their student groups. Data Collection and Analysis: Pre- and post-training assessments were conducted using the Revised Facts on Suicide Quiz and the Willingness to Intervene against Suicide Questionnaire. Repeated measures analyses assessed changes in these variables before and after training. Results: The study revealed a significant improvement in students' overall scores on the Revised Facts on Suicide Quiz and the Willingness to Intervene against Suicide Questionnaire post-training. Attitudes were not a significant predictor of intention to intervene; instead, subjective norms and perceived behavioral control emerged as significant predictors both pre- and post-training. Conclusions: These findings underscore the importance of training nursing assistant students in suicide prevention, and highlight the effectiveness of flexible training in improving their knowledge and intervention willingness. The findings can inform future strategies for enhancing nursing assistant students’ knowledge and willingness to address suicidal behavior.